herpes simplex virus type 1 hsv 1 strain kos 189  (ATCC)

Journal: Environmental Microbiology Reports

Article Title: Compost Tea as a Natural Bioactive Solution: Unlocking the Antimicrobial and Antiviral Potential of Bell Pepper and Citrus Wastes

doi: 10.1111/1758-2229.70260

Figure Lengend Snippet: Antiviral activity of CTs against enveloped viruses. Two assays are reported here. (A) Co‐treatment assay against HSV‐1 and RSV in (B); (C) virus pre‐treatment assay against HSV‐1 and RSV in (D). For HSV‐1, rhamnolipid M15RL (50 μg/mL) was used in the co‐treatment and virus pre‐treatment (Giugliano et al. ); for RSV, Hylin‐a1 (50 μM) was used in co‐treatment and virus pre‐treatment assays (Chianese et al. ). Untreated but infected cells were used as controls (ctr−). The data represent the mean ± standard deviation (SD) of three independent experiments. **** p ‐value < 0.0001; * p ‐value < 0.03; ns: not significant.

Article Snippet: The viruses used were Herpes Simplex Virus type 1 (HSV‐1) strain SC16, Respiratory Syncytial virus (RSV) (ATCC VR‐1540, Manassas, Virginia, United States), Poliovirus Type 1 (PV‐1) strain Chat (ATCC VR‐1562, Manassas, Virginia, United States), propagated on Vero‐76 cells (Cometa et al. ).

Techniques: Activity Assay, Virus, Infection, Standard Deviation

Journal: Frontiers in Pharmacology

Article Title: Characterization of the biodistribution profile of a human Dialyzable Leukocyte Extract (hDLE) by in vivo fluorescence imaging: a strategy to infer the ADME profile of complex multipeptide drugs

doi: 10.3389/fphar.2025.1701647

Figure Lengend Snippet: The hDLE peptides maintain their biological activity after conjugation with Alexa Fluor 488. (A) The m/z mass spectrometry profile of hDLE (blue spectra) and hDLE-Alexa (red spectra) showed that the ionization increases after conjugation because hDLE peptides acquire the ionization groups of Alexa Fluor 488 ( in set ), such as two -NH 2 . (B) The activity of hDLE-Alexa peptides was tested in a murine HSV-1 infection model. Observational (not infected/not treated) and infection (infected/not treated) controls showed 100% and 40% survival, respectively. hDLE, hDLE-Alexa, and the lyophilization hDLE control showed a Δ% survival rate of ≥40% compared to the infection control. All treatments were orally administered (0.75 μg) five times per mouse after HSV-1 infection. An additional infected group was orally administered with Alexa Fluor 488 (Alexa Fluor control), and no statistical differences were observed compared to the infection control. The Kaplan-Meier and post hoc Log-rank (Mantel-Cox) tests were performed to determine statistical significance; * p ≤ 0.05, ** p < 0.01; n = 10.

Article Snippet: Immediately after, mice were infected with 10-μL of 1.1 × 10 8 plaque-forming units (PFU)/mL of HSV-1 (KOS strain ATCC, VR-934) by scarification.

Techniques: Activity Assay, Conjugation Assay, Mass Spectrometry, Infection, Lyophilization, Control